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anti phosphorylated syk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti phosphorylated syk
    Anti Phosphorylated Syk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+syk/Phospho-Syk+(Tyr525%2F526)+Rabbit+mAb/pm42810528-62-43-47
    Average 95 stars, based on 223 article reviews
    anti phosphorylated syk - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Microglial TLR4-Lyn kinase is a critical regulator of neuroinflammation, Aβ phagocytosis, neuronal damage, and cell survival in Alzheimer's disease.
    Article Snippet: .. After blocking membrane was incubated with mouse anti-TLR4 (1:1000, Invitrogen, Cat# 14– 9041-80), rabbit anti-phospho-Lyn (1:1000 Bioss, Cat# bs-3257R), rabbit anti-Lyn (1:1000, CST, Cat# 2796S), rabbit anti-phospho-Syk (1:1000, CST, Cat# 2710), rabbit anti-Syk (1:1000, CST, Cat# 2712), rabbit anti-pi3k (1:1000, CST, Cat# 4292), rabbit anti-Akt (1:1000, CST, Cat# 4691), rabbit anti-phospho-Akt (1:1000, CST, Cat# 4060), rabbit anti-phosho-GSK3β (1:1000, CST, Cat# 9323), rabbit anti-GSK3β (1:1000, CST, Cat# 12,456) and rabbit anti-GAPDH (1:1000, CST, Cat# 2118S), antibodies. .. After overnight incubation at 4 °C with primary antibodies, membranes were washed thrice in 0.1%TBST and incubated with goat anti-rabbit IgG (1:20,000, Invitrogen, Cat# 31,460) and goat anti-mouse IgG (1:20,000, Invitrogen, Cat# 31,430) ECL secondary antibodies for 1 h at RT.

    Article Title: Microglial TLR4-Lyn kinase is a critical regulator of neuroinflammation, Aβ phagocytosis, neuronal damage, and cell survival in Alzheimer’s disease
    Article Snippet: .. After blocking membrane was incubated with mouse anti-TLR4 (1:1000, Invitrogen, Cat# 14–9041-80), rabbit anti-phospho-Lyn (1:1000 Bioss, Cat# bs-3257R), rabbit anti-Lyn (1:1000, CST, Cat# 2796S), rabbit anti-phospho-Syk (1:1000, CST, Cat# 2710), rabbit anti-Syk (1:1000, CST, Cat# 2712), rabbit anti-pi3k (1:1000, CST, Cat# 4292), rabbit anti-Akt (1:1000, CST, Cat# 4691), rabbit anti-phospho-Akt (1:1000, CST, Cat# 4060), rabbit anti-phosho-GSK3β (1:1000, CST, Cat# 9323), rabbit anti-GSK3β (1:1000, CST, Cat# 12,456) and rabbit anti-GAPDH (1:1000, CST, Cat# 2118S), antibodies. .. After overnight incubation at 4 °C with primary antibodies, membranes were washed thrice in 0.1%TBST and incubated with goat anti-rabbit IgG (1:20,000, Invitrogen, Cat# 31,460) and goat anti-mouse IgG (1:20,000, Invitrogen, Cat# 31,430) ECL secondary antibodies for 1 h at RT.

    Membrane:

    Article Title: Microglial TLR4-Lyn kinase is a critical regulator of neuroinflammation, Aβ phagocytosis, neuronal damage, and cell survival in Alzheimer's disease.
    Article Snippet: .. After blocking membrane was incubated with mouse anti-TLR4 (1:1000, Invitrogen, Cat# 14– 9041-80), rabbit anti-phospho-Lyn (1:1000 Bioss, Cat# bs-3257R), rabbit anti-Lyn (1:1000, CST, Cat# 2796S), rabbit anti-phospho-Syk (1:1000, CST, Cat# 2710), rabbit anti-Syk (1:1000, CST, Cat# 2712), rabbit anti-pi3k (1:1000, CST, Cat# 4292), rabbit anti-Akt (1:1000, CST, Cat# 4691), rabbit anti-phospho-Akt (1:1000, CST, Cat# 4060), rabbit anti-phosho-GSK3β (1:1000, CST, Cat# 9323), rabbit anti-GSK3β (1:1000, CST, Cat# 12,456) and rabbit anti-GAPDH (1:1000, CST, Cat# 2118S), antibodies. .. After overnight incubation at 4 °C with primary antibodies, membranes were washed thrice in 0.1%TBST and incubated with goat anti-rabbit IgG (1:20,000, Invitrogen, Cat# 31,460) and goat anti-mouse IgG (1:20,000, Invitrogen, Cat# 31,430) ECL secondary antibodies for 1 h at RT.

    Article Title: Microglial TLR4-Lyn kinase is a critical regulator of neuroinflammation, Aβ phagocytosis, neuronal damage, and cell survival in Alzheimer’s disease
    Article Snippet: .. After blocking membrane was incubated with mouse anti-TLR4 (1:1000, Invitrogen, Cat# 14–9041-80), rabbit anti-phospho-Lyn (1:1000 Bioss, Cat# bs-3257R), rabbit anti-Lyn (1:1000, CST, Cat# 2796S), rabbit anti-phospho-Syk (1:1000, CST, Cat# 2710), rabbit anti-Syk (1:1000, CST, Cat# 2712), rabbit anti-pi3k (1:1000, CST, Cat# 4292), rabbit anti-Akt (1:1000, CST, Cat# 4691), rabbit anti-phospho-Akt (1:1000, CST, Cat# 4060), rabbit anti-phosho-GSK3β (1:1000, CST, Cat# 9323), rabbit anti-GSK3β (1:1000, CST, Cat# 12,456) and rabbit anti-GAPDH (1:1000, CST, Cat# 2118S), antibodies. .. After overnight incubation at 4 °C with primary antibodies, membranes were washed thrice in 0.1%TBST and incubated with goat anti-rabbit IgG (1:20,000, Invitrogen, Cat# 31,460) and goat anti-mouse IgG (1:20,000, Invitrogen, Cat# 31,430) ECL secondary antibodies for 1 h at RT.

    Incubation:

    Article Title: Microglial TLR4-Lyn kinase is a critical regulator of neuroinflammation, Aβ phagocytosis, neuronal damage, and cell survival in Alzheimer's disease.
    Article Snippet: .. After blocking membrane was incubated with mouse anti-TLR4 (1:1000, Invitrogen, Cat# 14– 9041-80), rabbit anti-phospho-Lyn (1:1000 Bioss, Cat# bs-3257R), rabbit anti-Lyn (1:1000, CST, Cat# 2796S), rabbit anti-phospho-Syk (1:1000, CST, Cat# 2710), rabbit anti-Syk (1:1000, CST, Cat# 2712), rabbit anti-pi3k (1:1000, CST, Cat# 4292), rabbit anti-Akt (1:1000, CST, Cat# 4691), rabbit anti-phospho-Akt (1:1000, CST, Cat# 4060), rabbit anti-phosho-GSK3β (1:1000, CST, Cat# 9323), rabbit anti-GSK3β (1:1000, CST, Cat# 12,456) and rabbit anti-GAPDH (1:1000, CST, Cat# 2118S), antibodies. .. After overnight incubation at 4 °C with primary antibodies, membranes were washed thrice in 0.1%TBST and incubated with goat anti-rabbit IgG (1:20,000, Invitrogen, Cat# 31,460) and goat anti-mouse IgG (1:20,000, Invitrogen, Cat# 31,430) ECL secondary antibodies for 1 h at RT.

    Article Title: Fostamatinib (R788), a spleen tyrosine kinase inhibitor, sensitizes pancreatic cancer cells to oncolytic vesicular stomatitis virus
    Article Snippet: .. Membranes were then incubated in TBS-T with 5% BSA or milk with 0.02% sodium azide and a 1:5,000 dilution of rabbit polyclonal anti-VSV antibodies (raised against VSV virions), a 1:1,000 dilution of rabbit anti-phospho-STAT1 (catalog number 9167S, Cell Signaling), a 1:1,000 dilution of rabbit anti-STAT1 (catalog number 14994T, clone D1K9Y, Cell Signaling), a 1:1,000 dilution of rabbit anti-phospho-STAT2 (catalog number PA5-97361clone p-Y690, Invitrogen), a 1:1,000 dilution of rabbit anti-STAT2 (catalog number 4594, Cell Signaling), a 1:1,000 dilution of rabbit anti-phospho-STAT3 (catalog number 9134P, clone Y705, Cell Signaling), a 1:1,000 dilution of mouse anti-STAT3 (catalog number 9139P, clone 124H6, Cell Signaling), rabbit anti-phospho-MAPK (catalog number 4370S,clone p-T202/Y204, Cell Signaling), a 1:1,000 dilution of rabbit anti-MAPK (catalog number 9102L Cell Signaling), rabbit anti-phospho-SYK (catalog number 2710Sclone p-Y525, Cell Signaling), a 1:1,000 dilution of rabbit anti-SYK (catalog number 13198S, Cell Signaling), a 1:1,000 dilution of rabbit anti-OAS2 (catalog number PA5-65422 Cell Signaling), a 1:1,000 dilution of rabbit anti-GAPDH (catalog number 1E6D9 Proteintech), 1:1,000 dilution of rabbit anti-phsopho-TBK1/NAK (catalog number 5483P, clone S172, Cell Signaling), and a 1:1,000 dilution of rabbit anti-MX1 (catalog number 13750-1-AP, Proteintech). .. Starbright Blue 700 goat anti-rabbit (Bio-Rad, 12004161) immunoglobulin G (IgG) fluorescent secondary antibodies at 1:5,000 dilutions were used for fluorescent western blotting detection using the Chemidoc MP imaging system from Bio-Rad.

    Article Title: Microglial TLR4-Lyn kinase is a critical regulator of neuroinflammation, Aβ phagocytosis, neuronal damage, and cell survival in Alzheimer’s disease
    Article Snippet: .. After blocking membrane was incubated with mouse anti-TLR4 (1:1000, Invitrogen, Cat# 14–9041-80), rabbit anti-phospho-Lyn (1:1000 Bioss, Cat# bs-3257R), rabbit anti-Lyn (1:1000, CST, Cat# 2796S), rabbit anti-phospho-Syk (1:1000, CST, Cat# 2710), rabbit anti-Syk (1:1000, CST, Cat# 2712), rabbit anti-pi3k (1:1000, CST, Cat# 4292), rabbit anti-Akt (1:1000, CST, Cat# 4691), rabbit anti-phospho-Akt (1:1000, CST, Cat# 4060), rabbit anti-phosho-GSK3β (1:1000, CST, Cat# 9323), rabbit anti-GSK3β (1:1000, CST, Cat# 12,456) and rabbit anti-GAPDH (1:1000, CST, Cat# 2118S), antibodies. .. After overnight incubation at 4 °C with primary antibodies, membranes were washed thrice in 0.1%TBST and incubated with goat anti-rabbit IgG (1:20,000, Invitrogen, Cat# 31,460) and goat anti-mouse IgG (1:20,000, Invitrogen, Cat# 31,430) ECL secondary antibodies for 1 h at RT.

    other:

    Article Title: The late-onset Alzheimer’s disease risk factor RHBDF2 is a modifier of microglial TREM2 proteolysis
    Article Snippet: The rabbit polyclonal anti-alpha-tubulin antibody was purchased from Abcam (1:2,000, ab4074).

    Article Title: SYK-dependent lipid handling in monocyte-derived macrophages governs functional recovery after spinal cord injury.
    Article Snippet: The corresponding secondary antibodies used were as follows: Alexa Fluor 488 (1:500, A-21208, A-21206, A-11055, Invitrogen, USA) and Alexa Fluor 555 (1:500, A-31572, A-48270, A21432, Invitrogen, USA).



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